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KMID : 0386319940270020025
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Korean Leprosy Bulletin
1994 Volume.27 No. 2 p.25 ~ p.34
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Effects of Mycobacterium leprae or Mycrobacterium leprae-specific antigen, phenolic glycolipid-1 on the production of nitric oxide from murine peritoneal macrophages
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Kim Jong-Gu
Park Seok-Don
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Abstract
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Mycobacterium lworae is an obligate inoacellular organism that is phagocytosed by the
cells of mononuclear phagocyte system and proliferstes within thens The mechanism by
which M. leprae resists destruction remains poorly understood. Recently, nitric oxide(NO)
has been shown to play an important role in macrophages-media-text microbicidal
capacity for a variety of intracellulsr pathogens. NO production is used as an indicator
of microbicidal function of macrophages.
Using murine peritoneal macmphages, we studied the production of NO Eom
macrophages phagocytosed of M. leprae phenolic glycolipid-1(PGL-1) for the purpose to
elucidate the pathogenesis of leprosy. Macrophages were incubated with dead M. leprae
or PGL-1 and then treated with interferon-gamma(IFN-γ) and/or tumor necrosis
factor-alpha(TNF-α). The released NO was measured spectrophotometrically at 544nm
following reaction with the Griess reagent.
M. leprae and PGL-1 failed to stimulate NO release. IFN-γ plus TNF-α markedly
stimulated macrophages phagocytosed of M. leprae or PGL-1 to secrete NO.
These data suggest that detective IFN-γ-depdendent generation of NO may be an
important immunologic detect in the development of leprosy.
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